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In other words, do R-1234yf systems use the same charging port design as R-134a?

Quick Couple Adapters

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No, they are not. According to this website:

Vehicles with R-1234yf have different service fittings than R-134a vehicles to prevent cross-contamination with different refrigerants.

You can purchase new couplers which will convert the old R-134a gauge manifolds to the new R-1234yf couplers.

enter image description here

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  • Funny how the size was changed to avoid cross-contamination but then adapters are available to enable just that
    – Zaid
    Commented Jun 2, 2018 at 13:08

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